1:100 mouse anti-human β6 integrin subunit antibody (Merck KGaA)
90
Structured Review
Merck KGaA
1:100 mouse anti-human β6 integrin subunit antibody
1:100 Mouse Anti Human β6 Integrin Subunit Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1%3A100+mouse+anti-human+%CE%B26+integrin+subunit+antibody/1+100+mouse+anti+human+%CE%B26+integrin+subunit+antibody/pmc11218202-80-56-62
Average 90 stars, based on 1 article reviews
1:100 Mouse Anti Human β6 Integrin Subunit Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1%3A100+mouse+anti-human+%CE%B26+integrin+subunit+antibody/1+100+mouse+anti+human+%CE%B26+integrin+subunit+antibody/pmc11218202-80-56-62
Average 90 stars, based on 1 article reviews
1:100 mouse anti-human β6 integrin subunit antibody - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680"
Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680
Journal: Cancer Cell International
doi: 10.1186/s12935-024-03417-y
Figure Legend Snippet: Analysis of αVβ6 expression. ( A ) ITGB6 proteomic expression profile in normal tissue and primary tumor (data from UALCAN) ( n = 71–108; *** p < 0.001; Two sample t test). ( B ) RT-qPCR analysis of ITGB6 mRNA expression; β-actin was used as housekeeping gene. The (αVβ6+) cell line HT-29 was used as reference ( n = 3–8; *** p < 0.001; One-way ANOVA followed by Tukey’s assay). ( C - D ) β6 integrin subunit protein expression assessed by Western-Blot; tubulin was used as a loading control ( n = 3–7; *** p < 0.001; One way ANOVA followed by Tukey’s assay). Data are presented as mean ± SEM
Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Control
Figure Legend Snippet: Multicellular tumor spheroid characterization. ( A ) Typical cryosection images of H10 and H5M5 spheroids after KI-67 immunohistochemistry staining (scale bar = 150 μm). ( B ) Typical immunofluorescence images of H10 and H5M5 spheroids cryosections stained with antibodies against αVβ6 integrin, cytokeratin 19, E-cadherin, vimentin, and fibronectin at day 4 post-seeding. Proteins of interest are in red and nuclei are in bleu after counterstaining with Vectashield-DAPI (scale bar = 100 μm at x40 and 40 μm at x100). ( C ) Relative quantification of immunofluorescence markers expression using ImageJ software ( n = 3–4; * p < 0.05, ** p < 0.01; Two sample t test). Data are presented as mean ± SEM
Techniques Used: Immunohistochemistry, Staining, Immunofluorescence, Expressing, Software
Related Articles
SDS Page:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Migration:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Membrane:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Incubation:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Expressing:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Quantitative RT-PCR:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Western Blot:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Control:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Immunohistochemistry:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Staining:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Immunofluorescence:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 Software:Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Article Snippet: 40 μg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 |